更新時(shí)間:2016-05-10
我司U14小鼠子宮頸癌細(xì)胞,產(chǎn)地美國(guó)ATCC細(xì)胞庫,所有出入庫細(xì)胞均經(jīng)過嚴(yán)格的細(xì)胞質(zhì)量檢測(cè),確保細(xì)胞無污染,符合檢測(cè)合格標(biāo)準(zhǔn)。訂購7~15 天內(nèi)即可發(fā)貨,采用快遞郵寄,確保細(xì)胞Z短時(shí)間內(nèi)送到客戶手中。
免費(fèi)咨詢:021-54720761
U14小鼠子宮頸癌細(xì)胞培養(yǎng)注意事項(xiàng):
1、實(shí)驗(yàn)進(jìn)行前,無菌室及無菌操作臺(tái)(laminarflow)以紫外燈照射30-60分鐘滅菌,以70%ethanol擦拭無菌操作抬面,并開啟無菌操作臺(tái)風(fēng)扇運(yùn)轉(zhuǎn)10分鐘后,才開始實(shí)驗(yàn)操作。
2、U14小鼠子宮頸癌細(xì)胞操作工作區(qū)域應(yīng)保持清潔及寬敞,必要物品,例如試管架、吸管吸取器或吸管盒等可以暫時(shí)放置,其它實(shí)驗(yàn)用品用完即應(yīng)移出,以利于氣流之流通。實(shí)驗(yàn)用品以70%ethanol擦拭后才帶入無菌操作臺(tái)內(nèi)。
3、小心取用無菌之實(shí)驗(yàn)物品,避免造成污染。勿碰觸吸管尖頭部或是容器瓶口,亦不要在打開之容器正上方操作實(shí)驗(yàn)。容器打開后,以手夾住瓶蓋并握住瓶身,傾斜約45°角取用,盡量勿將瓶蓋蓋口朝上放置桌面。
3、工作人員應(yīng)注意自身之安全,須穿戴實(shí)驗(yàn)衣及手套后才進(jìn)行實(shí)驗(yàn)。
目錄號(hào) | CC-Y2098 |
細(xì)胞英文(簡(jiǎn)稱) | U14 |
細(xì)胞名稱 | |
背景資料 | 將U14瘤株腹腔接種于615 小鼠,利用腹水進(jìn)行原代培養(yǎng),建立細(xì)胞系。615小鼠、C57BL/6 小鼠移植成瘤率均為100%。 |
細(xì)胞來源 | ATCC |
代次 | P3 |
規(guī)格 | T25 |
細(xì)胞數(shù) | 50-100萬 |
價(jià)格 | 電議 |
生物安全級(jí)別 | 2 |
組織來源 | 宮頸癌 |
細(xì)胞形態(tài) | 貼壁;上皮細(xì)胞樣 |
細(xì)胞活力 | 95%(Viability by Trypan Blue Exclusion) |
細(xì)胞檢測(cè) | 細(xì)胞不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌 |
培養(yǎng)條件 | RPMI-1640 +10% FBS;37℃,5% CO2 |
傳代方法 | 建議1:2-1:3 兩天換液一次 |
凍存條件 | *培養(yǎng)基50%+40%FBS+10%DMSO |
提供的細(xì)胞類型有細(xì)胞系,每個(gè)細(xì)胞發(fā)貨前都必須經(jīng)過實(shí)驗(yàn)室QC檢測(cè),確保每個(gè)批次的質(zhì)量。是上海生物行業(yè)*的代理供應(yīng)商,提供報(bào)價(jià)、咨詢及技術(shù)服務(wù),選購。
我司其他相關(guān)銷售產(chǎn)品:
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Cs-(019)-002380 AN3 CA細(xì)胞株/細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002381 KLE細(xì)胞株/細(xì)胞,人子宮內(nèi)膜腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002382 HeLa細(xì)胞株/細(xì)胞,人宮頸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002383 Hela細(xì)胞株/細(xì)胞,人Hela細(xì)胞株/細(xì)胞耐藥亞株(Hela/DDP) ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002384 HeLa229細(xì)胞株/細(xì)胞,人宮頸癌細(xì)胞株/細(xì)胞(HPV-18永生化細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002385 Hela P10s-11F細(xì)胞株/細(xì)胞,人宮頸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(019)-002428 HuT 78細(xì)胞株/細(xì)胞,人T細(xì)胞株/細(xì)胞白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(019)-002430 THP-1細(xì)胞株/細(xì)胞,人單核細(xì)胞株/細(xì)胞白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002431 CHMAS細(xì)胞株/細(xì)胞,人肥大細(xì)胞株/細(xì)胞白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002432 Dami細(xì)胞株/細(xì)胞,人巨核細(xì)胞株/細(xì)胞保白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(019)-002433 Mo7e細(xì)胞株/細(xì)胞,人巨細(xì)胞株/細(xì)胞白血病細(xì)胞株/細(xì)胞株 ,規(guī)格: >5×100000cells/瓶
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