更新時間:2016-06-03
我司所提供的實(shí)驗(yàn)KLN205:鼠肺磷癌細(xì)胞,不能用于人體實(shí)驗(yàn)和臨床診斷、治療。我司細(xì)胞研究中心承諾,盡Z大努力對實(shí)驗(yàn)細(xì)胞資源相關(guān)信息進(jìn)行及時更新,同時也保證純度在90%以上,咨詢購買。
免費(fèi)咨詢:021-54720761
KLN205:鼠肺磷癌細(xì)胞培養(yǎng)方法:
收到細(xì)胞后,在倒置顯微鏡下觀察整個細(xì)胞生長情況,如果細(xì)胞未長滿,用75%酒精噴灑整個瓶消毒后放到超菌臺內(nèi),嚴(yán)格無菌操作,打開細(xì)胞培養(yǎng)瓶,留10ml培養(yǎng)液繼續(xù)培養(yǎng)。
KLN205:鼠肺磷癌細(xì)胞初代培養(yǎng)原理:
將動物機(jī)體的各種組織從機(jī)體中取出,經(jīng)各種酶(常用胰蛋白酶)、螯合劑(常用EDTA)或機(jī)械方法處理,分散成單細(xì)胞,置合適的培養(yǎng)基中培養(yǎng),使細(xì)胞得以生存、生長和繁殖,這一過程稱原代培養(yǎng)。
選購流程:
采購實(shí)驗(yàn)裝備就是如此簡單。我司細(xì)胞活力狀態(tài)良好的細(xì)胞株1420多株,細(xì)胞來源可靠,背景資料清晰,代數(shù)年輕,活力好。其中絕大部分是近年來從美國ATCC和NIH分批引進(jìn)的細(xì)胞種子,少部分來自全國各地細(xì)胞研究所。
從實(shí)驗(yàn)室的設(shè)計到實(shí)驗(yàn)過程中的操作,都需要嚴(yán)格控制無菌。由于細(xì)胞對于生長的條件比較苛刻,所以無論從實(shí)驗(yàn)室的設(shè)計,還是使用的物品及細(xì)胞培養(yǎng)的操作過程,都要注意保持無菌環(huán)境。
我司特性經(jīng)測試:具有良好的增殖和分化潛能,可分化為成骨細(xì)胞、脂肪細(xì)胞、軟骨細(xì)胞等。流式檢測結(jié)果顯示,CD29、CD44、CD73、CD105、CD166陽性,CD11a、CD34、CD45陰性。細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無機(jī)離子及其他一些入核酸降解物、激素等。
我司其他相關(guān)批發(fā)產(chǎn)品展示:
Cs-(025)-002909 SUNE-1亞株-5-8F細(xì)胞株/細(xì)胞,鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002910 F2-2細(xì)胞株/細(xì)胞,5-8轉(zhuǎn)基因鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002911 SUNE-1亞株6-10B細(xì)胞株/細(xì)胞,人鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002912 SUNE-1亞株13-9B細(xì)胞株/細(xì)胞,人鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002913 HNE2-LMP1/2細(xì)胞株/細(xì)胞,人鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002914 HNE2-LMP1細(xì)胞株/細(xì)胞,人鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002915 SUME-a細(xì)胞株/細(xì)胞,人鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002916 FD4細(xì)胞株/細(xì)胞,人淋巴細(xì)胞株/細(xì)胞與倉鼠肺細(xì)胞株/細(xì)胞雜交細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002917 FD6細(xì)胞株/細(xì)胞,人淋巴細(xì)胞株/細(xì)胞與倉鼠肺細(xì)胞株/細(xì)胞雜交細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002918 NCI-H446細(xì)胞株/細(xì)胞,人小細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002919 NCI-H345細(xì)胞株/細(xì)胞,人小細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002920 DMS153細(xì)胞株/細(xì)胞,人小細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002921 NCI-H1688細(xì)胞株/細(xì)胞,人經(jīng)典小細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002922 NCI-H460細(xì)胞株/細(xì)胞,人大細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002923 NCI-H661細(xì)胞株/細(xì)胞,人大細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002924 NCI-H520細(xì)胞株/細(xì)胞,人肺鱗癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002925 NCI-H1650細(xì)胞株/細(xì)胞,人肺支氣管癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002926 NCI-H157細(xì)胞株/細(xì)胞,人非小細(xì)胞株/細(xì)胞肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002927 NCI-H1975細(xì)胞株/細(xì)胞,人非小細(xì)胞株/細(xì)胞肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002928 NCI-H838細(xì)胞株/細(xì)胞,人非小細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002929 NCI-H2227細(xì)胞株/細(xì)胞,人肺小細(xì)胞株/細(xì)胞肺癌 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002930 NCI-H292細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞(淋巴結(jié)轉(zhuǎn)移) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002931 NCI-H596細(xì)胞株/細(xì)胞,人肺腺鱗癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002932 NCI-H23細(xì)胞株/細(xì)胞,人非小細(xì)胞株/細(xì)胞肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002933 NCI-H2087細(xì)胞株/細(xì)胞,人非小細(xì)胞株/細(xì)胞肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002934 A-427細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002935 H125細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002936 PG細(xì)胞株/細(xì)胞,人肺巨細(xì)胞株/細(xì)胞癌 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002937 A549/DDP細(xì)胞株/細(xì)胞,耐DDP肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002938 QG-56細(xì)胞株/細(xì)胞,人肺扁平上皮癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002939 95-D細(xì)胞株/細(xì)胞,人高轉(zhuǎn)移肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002940 801-D細(xì)胞株/細(xì)胞,人巨細(xì)胞株/細(xì)胞性肺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002941 SPC-A1細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002942 LTPEP-a-2細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002943 LTEP-a-2細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002944 H1299細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002945 SK-MES-1細(xì)胞株/細(xì)胞,人肺鱗癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002946 Calu-3細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞(胸膜滲出液) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002947 Calu-6細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞系(未分化) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002948 HLF-02細(xì)胞株/細(xì)胞,人胚肺二倍體細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002949 Tul-1細(xì)胞株/細(xì)胞,人肺癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002950 HFL-I細(xì)胞株/細(xì)胞,人胚肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002951 MRC-5細(xì)胞株/細(xì)胞,人胚肺細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002952 WI-38細(xì)胞株/細(xì)胞, 人胚肺細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002953 WI-38AV13細(xì)胞株/細(xì)胞,人SV-40轉(zhuǎn)化肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002954 PLC/PRF/5細(xì)胞株/細(xì)胞,人肺癌亞歷山大細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002955 PG-LH7細(xì)胞株/細(xì)胞,人肺巨細(xì)胞株/細(xì)胞癌(PG)的低轉(zhuǎn)移亞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002956 PG-BE1細(xì)胞株/細(xì)胞,人肺巨細(xì)胞株/細(xì)胞癌(PG)的高轉(zhuǎn)移亞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002957 PG49細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002958 GLC-82細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002959 HLAmP細(xì)胞株/細(xì)胞,人肺巨細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-002960 GLC-82-TK細(xì)胞株/細(xì)胞,人肺腺癌細(xì)胞株/細(xì)胞(轉(zhuǎn)染TK基因的GLC-82細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
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